BIOSC 0160 Chapter Notes - Chapter Chapter 13: Maurice Wilkins, Frederick Griffith, Deoxyribonuclease
Document Summary
1. experimental evidence showing dna is the transforming principle : the structure of dna, difference between an exonuclease and endonuclease, experimental evidence on how dna replicates. Dna was in the nucleus along with the stained chromosome that were known to carry genes. Identified bacteria can genetically change cells to become virulent. Discovered what was causing the genetic change: dna. Bacteriophage-made of protein and dna (only dna enter cell) Labeled cysteine/methionine with s35 sulphur and dna with p32 phosphate. Agitated cells and dna was in the cell but protein in the supernatant fluid. Sugar-phosphate backbone at the outside of the helix. 60%a/t and 40% g/c: what percentage of g if a is 20%? (100-20x2)/2=30% Sugar phosphate backbone held by phosphodiester bond with nucleotide (bases) held by h-bond. Antiparallel to reverse the polarity to form h-bond. Strand has a phosphate at the 5" and the opposite has a hydroxyl at the. Bases in the major groove are more accessible than the minor grooves.
