BIOL 1107 Chapter Notes - Chapter 20: Molecular Cloning, Chain Termination, Genetic Engineering
Document Summary
First human genome sequence took several years 1 billion dollars. Today to sequence a human genome it takes 6 hours and cost . Dna technology: techniques for sequencing and manipulating dna. Affects agriculture to criminal law and medical research. Biotechnology: manipulation of organisms or components to make products. Dates back to agricultures dawn of civilization. Are valuable tools for genetic engineering and biological inquiry. Nucleic acid hybridization: base pairing of one strand of nucleic acid to complementary sequence on strand from different nucleic acid molecule. Genetic engineering: manipulation of genes for practical purposes. Used to modify genes and move between organisms. Dna sequencing: determines nucleotide sequence of dna molecule. Dna cut into fragments each fragment is sequenced. Dideoxyribonucleotide chain termination sequencing: one strand of dna fragment is used as template to create set of complementary fragments. Next-generation sequencing: machine sequences 70-90 million nucleotides per hour. Results: each well hold different dna fragment different sequence;
