CHEN 3701 Lecture Notes - Lecture 10: Mpra Language, Opata Language, Delaware Route 1
28 Sep 2015
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This is just a specific example of uncompetitive inhibition. Just now, the inhibitor is also the subsrate: rate, material balance, rapid eqbm: this = general uncompetitive inhibition rate of rxn, rearrange to look like substrate ihibition. Chen3701_biomolecularengineering page 1 take derivative of the rate. @ high t, enzyme is unstable and denatures if we assume arrhenius form, Methods: adsorption - attach enzymes on the surface by weak forces (van der waals) Desorption can reduce performance (b/c have less enzyme there than before) covalent bonding - via functional groups (amino acids, carboxy, hydroxyl, non-natural) Needs to be distant from active site. [s]bulk also depends on hydrodynamics: conditions around the material and how well distributed is the enzyme (surface, throughout the particle) This is all described by the damkholer number (da) If da >> 1, mass transfer is rate-limiting. At steady state mass transfer rate = reaction rate vmax" = per surface area whereas other rates are per volume.
