AGRICULTURE MICROBIOLOGY Lecture Notes - Bacterial Conjugation, Lysogenic Cycle, Hfr Cell

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19 Oct 2022
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The process of transfer of genetic material and recombination is very interesting bascterial recombination is given (ppt. an overview of bacterial recombination). the three main mechanisms by which bacteria acquire new dna are transformation, conjugation, and transduction. Transformation involves acquisition of dna from the environment, conjugation involves acquisition of dna directly from another bacterium, and transduction involves acquisition of bacterial dna via a bacteriophage intermediate. Transformation is the process by which bacteria pick up dna from their environment. The dna may come from a variety of sources, but most likely it is the remnants of dna from dead bacterial cells. In order to become successfully transformed, bacteria must be competent. This means that the bacteria are expressing the appropriate enzymes (the "transformation machinery") required to transport the exogenous dna into the cell. Therefore, the correct genes must be expressed in order to carry out transformation. In transformation, a cell surface receptor binds to dna in the environment.

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