Biology 3316A/B Lecture Notes - Lecture 14: Beta-Catenin, Cilium, Catenin
Document Summary
Lecture 14 in situ hybridization and hh signalling. Can get an idea of where the gene is expressed usig in situ hybrodization: now you can find ways of tweaking it. Can use it on tissue sections: fixed tissues. Can make the probes fluorescence: this is called fish. Looking at rna; an rna-rna hybrid is very stable: we use dig because it is a plant protein that is specific to the plant antibody. Since you"re looking at mrna, this gives you no indication of signalling: wnts, hh are both produced in one place and then gets secreted as a protein, all its telling you is where the original signal was expressed. Dig labeled to utp or some molecule that is attached to a nucleotide. In this case, it is rna because a rna-rna bond is very stable. Go from a colourless solution to an insoluble purple colour.
