MD 1010:12 Lecture 8: TCA Cycle Summary
-10801kWh :
fluovoaietate
µl0A
Acetyl Lott
\CoA 7flnorocitrate
flnoroaietyl -WA ✓(inhibits aconitase )
citrate synthase
/
↳
oxaloacetate citrate
7t
malate (
NA ""2 citrate isomerase
dehydrogenase (aconitase )
NAD it
d
malate isocitrate
TNAD I
fumerase ~
*sensitive to
[ATP ]isocitrate
\Hz0 NADHZ }dehydrogenase
(02
m
merate K
TNFADHZ a-ketoglutavate
CoA
succinate NAD /
dehydrogenase (Fap
#c- ketoglutarate
*malonate is acompetitive >succinyl .CoA NADTK dehydrogenase *
L
inhibitor succinate ,synthetase (02
A
snccinyicon .
LV
LOZ ATP ADPTPI
*a- ketoglutavate dehydrogenase
EI :regulated by ATP
EL :regulated
byloalsuccinyl-CoA
E3 :regulated by NADINADHZ
v
snccinyltoatpi -succinyl -Pit (OA
(snccinyl -Pit GDP -snccinate -1 GTP ]
. The TC Acycle completes the oxidation of what REACTION #3:
remains of the glucose that was converted to . This reaction is catalysed by Iso citrate
pyvuvate .dehydrogenase,is0Citrate Is both decarboxylated
↳transported into the mitochondria and then and oxidised .
converted to Acetyl CoA .
↳so acarbon is removed from iso citrate To form
'In the TCA cycle ,Acetyl CoA is oxidised and a-Ketoglutarate .
decarboxylated and CoA recycled producing .In this reaction adehydrogenase is involved
,mean -
reduced electron carriers and carbon dioxide .ing ,NAD is converted to NADH .
REACTION #1:Iso citrate dehydrogenase
.Acetyl 10A (2C )merges with Oxalo acetic acid
(02
loxalo acetate )14C )to produce citrate (64 .This isocitrate /)a-Keto -
reaction is catalysed by the enzyme ,citrate ~glutamate
synthase .NAD NADH
Acetyl 10A
\CoA REACTION #4:
OXAIO acetate -Citrate .During this reaction acarbon Is taken from a.
citrate synthase Ketoglutarate to produce snccinyl LOA .
this reaction is catalysed by the enzyme ,
a-
Ketoglutarate dehydrogenase
REACTION #2:↳Dehydrogenase enzyme :results in decarbo -
.Involves the reorganisation of the atoms within oxylation and the conversion of NAD To NADH .
the citrate to form iso citrate Ian isomer )of
citrate
. The enzyme that catalyses this reaction is an a-ketogluterate
Isom erase ,citrate isomevase .However this enzyme dehydrogenase
is referred to as aconitase .
(02
.
citrate -noo ,.+ra+e
"ke +09 mterate #snccinyl con .
aconitase NAD (0A NADH
REACTION #5:
.The reaction involves the synthesis of anucleoside
triphosphate GTP rather than ATP .
. The reaction Is catalysed by the enzyme ,
Document Summary
Succinyl coa so continue with on the enzyme does not need to the. Adp however can all ostevically enzyme . levels of. Adp means that there is a lot breakdown. Is high regulated because the reaction it catalyses is irreversible. Metabolism this means that there is occurring in our body , are high levels of energy production . It a lot of atp is made it can act as an allosteni inhibitor for this enzyme . " high levels of nadh can also allostenially inhibit this enzyme . In the tca cycle , a lot of. Nadh inhibitor is being by energy lot of an. Is a and that we do not produced indicating production and it act as that there occurring need anymore . # 2 : aconitase citrate - iso reaction is isomerazation enzyme the that. Alonitase reaction . citrate reaction . catalyses this. Is usually the under tatty it goes pathway . there.
