BIOM30001 Lecture Notes - Lecture 9: Macrophage, Systems Biology, Adenylate Kinase
Metabolome (6,862 metabolite peaks)
○
Autoantibodies
○
Total of 2 billion measurements taken over 20 time points
○
iPOP provides multidimensional view of medical state (including healthy state)
•
Michael had a genetic predisposition to T2D, which was triggered by flu episodes, but
avoided due to changes in diet and drug therapy
○
Disease risks inferred from genome can be assessed by monitoring traits to identify changes in
physiology
•
Longitudinal study highlight changes that may not be picked up in population studies.
•
Potential use in health monitoring, diagnosis, prognosis and treatment
•
Cost a major issue
•
Tissue imaging mass spectrometry
Performs MS on each 'pixel' of the sample
•
Able to visualise where metabolites are
•
Rapid evaporative ionisation MS (REIMS) is being tested for real-time (intraoperative)
monitoring of human tumour tissue
•
Electrosurgical dissection results in a fine 'smoke' that can be analyzed directly by MS
•
The iKnife allows detection of hundreds of metabolites and processing within a few seconds
(compared to 30 min for normal histology during)
•
Intra-operative mass spectrometry : the iKnife (intelligent knife)
Frontiers Page 8
(compared to 30 min for normal histology during)
Allows surgeon to see if there is any more tumour tissue left
•
Mass spectra of tumour and different surrounding tissues
•
Tissue ID in intraoperative REIMS matched post-operative histology in 100% of cases
•
Real time analysis of data (multivariate analyses) within 0.7 sec. Not using biomarkers –
looking at entire spectrum !
•
Demonstrated utility for breast cancer, neurosurgury (gliomas) use irrigating bipolar forceps,
identification of bacteria (20,000 different strains typed)
•
Measuring metabolic dynamics in vivo - flux
Frontiers Page 9
Document Summary
Total of 2 billion measurements taken over 20 time points ipop provides multidimensional view of medical state (including healthy state) Disease risks inferred from genome can be assessed by monitoring traits to identify changes in physiology. Michael had a genetic predisposition to t2d, which was triggered by flu episodes, but avoided due to changes in diet and drug therapy. Longitudinal study highlight changes that may not be picked up in population studies. Potential use in health monitoring, diagnosis, prognosis and treatment. Performs ms on each "pixel" of the sample. Intra-operative mass spectrometry : the iknife (intelligent knife) Rapid evaporative ionisation ms (reims) is being tested for real-time (intraoperative) monitoring of human tumour tissue. Electrosurgical dissection results in a fine "smoke" that can be analyzed directly by ms. The iknife allows detection of hundreds of metabolites and processing within a few seconds (compared to 30 min for normal histology during) Frontiers page 8 (compared to 30 min for normal histology during)
