BIOM30001 Lecture Notes - Lecture 23: Bleomycin, Flow Cytometry, Bronchus
Document Summary
23b: lung stem cell therapy for respiratory diseases. Challenges in using stem cells for lung therapies: complexity of tissue, diversity of disease. First example of spontaneous compensatory lung growth in humans. Lineage tracing: cells expressing a gene are modified so when that cell expresses that gene it expresses a marker (e. g. gfp). Allows identification of which cells are expressing the gene of interest during embryonic development. Knockout/knock-in mouse models: to see what happens after ko. Can then tell which cells are responsible for the regenerative process. During branching morphogenesis there is an interaction of endothelial and mesenchymal progenitors. Mesenchymal progenitors secrete fgf10, which has a chemotactic effect on epithelial stem cells > they elongate towards the mesenchyme. These mature and differentiate to form mature epithelial and mesenchymal stem cells. Duct cells: niche of progenitor cells in the submucosal gland. Basal cells: intercartilaginous regions in trachea and upper airway, give rise to progenitor cells.
