GENE20001 Lecture 22: 22 Mitotic cell genetics A
Document Summary
Somatic cell genetics can be used to map genes to chromosomes. The nature of the variation used in these experiments is usually biochemical and involves ways in which we can identify the protein products of genes. However the variation we are interested in is between human and mouse. Electrophoresis involves the separation of proteins through a gel matrix (agar/acrylamide gel) under the influence of a voltage potential. The proteins are separated on the basis of their size and charge. The gel is then stained for total protein and for a specific enzyme/protein (activity or antigenicity). The first lane is for all the proteins found in a mouse cell. In lane 2 the human cell has a similar pattern. In the other lanes an antibody is used that can identify a protein that is in both human and mouse. In this example the human protein moves further through the gel than the mouse.
