GENE20001 Lecture Notes - Lecture 20: Centromere, Case Blue, Lysogenic Cycle
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Consider a locus: group 1, 2, 3 are parental in terms of a locus. Divide by two since only half the chromatids are actually recombinant. Compare this to the previous experiment were we worked out distance between a and b was 9. 3-5. 05=4. 25. This is because there may be multiple crossovers that we don"t see as the distance between a and b increase: this is a complementation test. We should see the mutant phenotype (clear plaques). Turbid plaques arise because recombination may occur which produces a wt. If the two mutations were in different genes then 20% would be turbid rather than just a few. Not c. this sort of thing happens in t4 but that was a little different. In this instance there shouldn"t be much genetic diversity. Mottled appearance means some progeny drive lytic and some drive lysogenic cycle. Here coinfection will produce clear plaques because mutants do not complement.
