BIOL3006 Lecture Notes - Lecture 2: Immunoglobulin Light Chain, Cell Membrane, Tyrosine
Document Summary
Microscopy: light microscopy using specific markers, em to identify earliest carriers, need recognition of cellular components/vesicles. Instead of being recycled back to the same surface or lysosome, additional trafficking takes vesicles across the other side of cell: eg. Ab transferred from milk to the bloodstream of a mother and vice versa. Insulin treatment, exocytosis increased, endocytosis unchanged, net translocation to. Pm: remove insulin, glu4 returns to compartment through clathrin coated endocytosis, type ii diabetes, cells insulin resistant, no insulin stimulation, no glut4 translocation, high blood glucose (glucose not internalised in cells) Involves multiple low affinity interactions between coat proteins, adaptors, cargo proteins and lipids. Components: heavy and light chains, high salt (coat released), low salt (coat reforms, spontaneous assembly to form a cage, energy processes needed in disassembly. Adaptins bind to r tails and clathrin. Involves lipids (phosphatidyl inositol crucial for different cell processes and there are different types on different membranes)
