MICR3002 Lecture Notes - Lecture 2: Antigen, Abacavir, T Helper Cell
Document Summary
Tat opens up promoter and recruits proteins to open nucleosomes in active conformation. When open, rna pol ii can move through. Tat becomes acetylated on its k50 domain, which tightens its binding with cyct1 (on ptefb complex), which in turn better binds to tar rna. Sirt1 is a hdac which can deacetylate k50 so tat no longer binds to tar rna. Hiv genome contains 9 major genes, which are derived from a single protein transcript. Many mrnas can be made by alternative splicing. Hiv mrna is made is two phases: early (approx. 4-5kb: undergoes double slicing, only occurs in enough concentration of rev, regulated by rre (which is present in long trancripts. Binds to the rre which is present on all singly spliced and unspliced mrna. Rev has a couple initial binding sites on rre where it can multimerise. Doubly spliced transcripts from the hiv genome are important ones: tat: regulates transcription, rev: regulates transport.
