MICR3002 Lecture Notes - Lecture 3: Small Interfering Rna, Retrotransposon, Fluorescence
Document Summary
Lecture 3 replication and rnai expression (+) ssrna plant virus lifecycle. Rna binding proteins (rbp) play critical roles in translation, replication and movement: viral and host protein encoded, host membrane targeting and reorganisation. Important for viral replication (as it doesn"t occur free floating in cytosol: assembly of viral replication complexes (vrc, structures associated with membrane, come together via viral and host proteins and membranes. Roles of host factors in biogenesis of vrc: viral replication doesn"t happen in solution, hidden away from host cell in pits , need to do this to get away from. Rna silencing defence machinery (-) ssrna plant virus lifecycle. Rhabdovirus: nucleorhabdovirus, replicates in nucleus, cytorhabdovirus, replicated in cytosol. Need 3 core proteins to become infectious. P protein (phosphoprotein: co-factor for replicase. G protein (glycoprotein - spikes: pick up from host. Lnyu protein localisation: free gfp localises to cell peripheries. Therefore, it can be assumed that viral proteins are usually localised to the periphery.
