BIOL1007 Lecture Notes - Lecture 3: Phosphodiester Bond, Deamination, Nucleoside
Document Summary
Identify the main chemical components of nucleic acids and their interactions involved in base pairing between nucleic acid strands. The distance they migrate depends on size: ethanol precipitation: nucleic acids become insoluble when mixed with salt (to neutralise charge) and ethanol, base pairings include: Nucleotide = a base + sugar + phosphate. You can monitor purity by checking ratios of absorbance values for likely contaminating molecules: hydrogen-bonding potential (donor/acceptor) Lecture 3: information in biopolymers nuclei acids: base pairing: C and g complement each other (3 hydrogen bonds so stronger binding) A and t/u complement each other (2 hydrogen bonds so a bit weaker: tm/melting point when 50% of the molecule is unfolded/separated. Long-term storage of genetic information; transmission of genetic information to make other cells and new organisms. Used to transfer the genetic code from the nucleus to the ribosomes to make proteins.
