ANHB3323 Lecture Notes - Lecture 21: Atomic-Force Microscopy, Mechanobiology, Rheology
3 Jun 2018
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LECTURE TWENTYONE: Tools for Mechanobiology
ECM to Nucleus:
• ECM properties/stiffness
o AFM
o Micro indentation
• Traction force
o TFM
o Micro-pillar
o FRET
Measurement Tools:
• Wettability
o Measuring hydrophilicity or hydrophobicity
o Higher angle → more hydrophibic
o Lower angle → more hydrophilic
• Topography
o More qualitative → determining structure
o Using SEM
o Limitation → depth
o Using technology → measuring fiber size
• Mechanical properties
o Macro scale
▪ Compression test
▪ Tensile test
▪ Rheology test
o Micro scale
▪ Microindentation/compression test
▪ Atomic force microscope
Uses for Atomic Force Microscope:
• Force
• Stiffness
• Height of contact → topography/roughness
• Adhesion force
• Rupture force/distance
• Conductivity
• DNA stretching
• Protein binding force
Measuring Intracellular Traction Force:
• Pulling force from cells
o Stiffer → more FAC → higher traction
o Less stiff → less FAC → lower traction
• Direct cellular measurement
o Surface area of micro pillar
o Size/distribution of beads
o Magnetic beads/field
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