FNSC2200 Lecture 11: 4. Drug Analysis
Document Summary
Gc vs hplc: advantages, disadvantages, stationary phase, mobile phase. Weakness in this type of analysis + advantage of drug cheats. Quantification: active species in sample & in subject. Hair, saliva, counterfeit drugs, blood, urine, chemical residues on clothing, solid/liquid samples of preliminary identified ilicit drugs. P6 simple chem tests for 1 drug identification. Separates complex and organic mixtures (cho, i. e. drugs) Suitable for samples that can be heated up. Mobile phase is non polar, stationary phase is polar slow. Generally for non-volatile samples, dont like to be heated. Takes complex mixtures (solid/liquid/gas, as long as dissolved in solvent) Expensive large amount of solvent needed, short column life. High level tech expertise is required to run and process. Sample must be volatile, must be put into gas-phase. Output from gc is treated with ms to get fp. Ms confirms peak on chromatogram corresponding to a specific chemical species. Use calibration curve: run conc of target species through.
