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BIOL 2000 Lecture Notes - Streptomycin, Karyotype, Threonine

Course Code
BIOL 2000
Joseph Rasmussen

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Chapter 5-2
Properties of the F-Factor (Plasmid)
Enables the production of pilli
o Proteniaceous attachment tube
between cells
Able to replicate during cell division and
during conjugation
o Will transfer the F plasmid to their
o F+ remains constant in the
o Each daughter cell will gain this F+
Prevents conjugation between two F+
o One must be F- for conjugation to occur
F Plasmids transfer during conjugation
Transfer of single-stranded DNA
o F+ is donor
o F- is recipient
o Only one direction of transfer
F+ will create the pillis
Replication in both donor and recipient
Rolling circle replication in donor
o F plasmid DNA is double stranded and circular
o The defined position at the origin will be cut
o Serves as a starting point for the transfer process
o Origin point is the first point to arrive in the F- cell
o The arrow head will indicate the direction of DNA transfer
The donor cell does not loose DNA
o DNA synthesis is replacing the DNA that is being transferred
o The recipient is also synthesizing the template, to create a double
stranded DNA molecule
DNA synthesis in both cells are ensuring both cells have F+ plasmid double
stranded DNA
F Plasmid DNA will only be transferred
o No other DNA will be transferred
Clicker question
During Bacterial conjugation, which one of the following events would not be
expected to occur?
o An F- cell would be converted to a F+ cell
o An F+ cell would generate pili to attach to F- cells
o DNA would be replicated in the F+ cell
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