CMMB 461 Lecture Notes - Lecture 31: Synthetic Genetic Array, Synthetic Lethality, Kanamycin A

• School: University of Calgary
• Department: Cellular, Molecular and Microbial Biology
• Course: CMMB 461
• Professor: Gordon Chua

Document Summary

Synthetic lethality identifies functional and buffering relationships. Large-scale synthetic lethality analysis: global map of functional relationships between genes and pathways. Single mutant: deletion of gene a, cell is still viable. Delete both genes: lethal synthetic lethality. This case tells that the two genes interaction is synthetic lethality. If c is synthetic to x, expect d to be synthetic to y. If you delete d and y, expect synthetic lethality, similar to ce and ez. You can also see if you delete cy and cz, that"ll be synthetic lethal. Anytime you delete 2 genes connected by line are synthetic lethal. This only occurs if genes belong to 2 functionally redundant pathways. If you delete c on its own, cell lives because xyz pathway can compensate loss of c (buffering) If you cross x with cde and it is synthetic lethal, then you know cde are in the same pathway but are separate from x.