ENVS 2210 Lecture Notes - Lecture 6: Old Queens, Royal Jelly, Artificial Cell

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Queen Rearing
Part of the information is contained in the textbook plus lecture material
I will emphasize the following:
WHY REAR QUEENS AND WHAT IS NEEDED
- Why rear and replace queens?
To produce more workers
Old queens lay less eggs than young queen
To obtain higher honey yields
More populated colonies to split sell nuc colonies, to pollination etc
To improve the health conditions of colonies
More populated = lower incidence of diseases and parasites
Must be done with genetically improved queens
Cannot just grab any queen to do it
Choose a queen from a colony that performs well
- What does it take to rear queens?: understanding the bees’ reproductive biology
Imitate natural conditions
Understand the bees’ reproductive biology
Acquire specific equipment designed to rear queens
Get some training
Acquire or select good bee stocks
Need to have records to select the best
- Natural production of queen bees
Queenless colony
Swarming
Queen supersedure
Because queen is not well, not producing enough queen substances
SIMPLE METHODS OF QUEEN REARING
- Queen removal
Produce queenless colony, then they have to rear a new queen
- Colony splitting (dividing the colony in two)
Division of one colony into two and one half have the queen, the other half does
not
- The Miller Method
- The Alley method
Introduce an empty comb into a breeding colony for 4 days
4 days, if the queen lays egg in that cell and the egg will be in embryo stage for 4
days and in 4 days the larvae will hatch
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After 4 days you retrieve that comb and cut cell strips with larvae
Destroy 1 of every 2 cells
Attach strip to the inferior edge of a comb
Introduce comb into a queenless colony for 10 days
10 days later, they will produce queen cells
Remove the comb and select cells
Introduce queen cells into queenless colonies or divisions
Good for beekeepers with few hives
- Doolittle method
Most widely used method worldwide
1) Grafting
2) Cell building
3) Harvest of queen cells
4) Mating and harvest of queens
THE COMMERCIAL (DOOLITTLE) METHOD
- Grafting
The transference of a larva from a comb cell to an artificial queen cells cup
Collect the larvae from cells from a good queen and transfer them to
artificial queen cups
The larvae should be from valuable queens
Selected, colonies performed well
Queens acquired from a good breeder
- Grafting materials
Artificial cell cups
Plastic or wax cell cups
Can be made by putting wax in molds
Diameter 8-9 mm
The wax cell cups are made with wooden molds
Easy to make a lot of them
The holding frame
Contains 2-3 wooden bars with about 15 cells cups per bar
May be introduced into a cell builder colony for a short time before
grafting
Very little science in queen rearing, so it remains the same for 100s of years
Not attractive for scientists to do research on
Low impact journal
- The grafting technique
Spoon the larva by its curved side from the cell
Touch the base of the cell cup with a downwards movement and leave the larva
at the bottom of the queen cup
Some like to put royal jelly mixture in the queen cup
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Document Summary

Part of the information is contained in the textbook plus lecture material. Old queens lay less eggs than young queen. More populated colonies to split sell nuc colonies, to pollination etc. To improve the health conditions of colonies. More populated = lower incidence of diseases and parasites. Must be done with genetically improved queens. Cannot just grab any queen to do it. Choose a queen from a colony that performs well. Acquire specific equipment designed to rear queens. Need to have records to select the best. Because queen is not well, not producing enough queen substances. Produce queenless colony, then they have to rear a new queen. Colony splitting (dividing the colony in two) Division of one colony into two and one half have the queen, the other half does not. Introduce an empty comb into a breeding colony for 4 days.

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