5-350 Lecture Notes - Interleukin 10, Trophoblast, Indolamines

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Fertilization, Preimplantation Development, Implantation
FERTILIZATION
Steps In fertilization
1. Penetrate Corona Radiata
- protein layer, hyluronic acid ECM
- requires sperm swimming (CatSper Ca++ channel)
- requires hyluronidase
2. Zona Pellucida binding
- glycoprotein coat around oocyte
- ZP3 binding
- sperm receptor:
species specificty
antibody blockage
no ZP3 binding of fertilized eggs
B-1,4 GALACTOSYLTRANFERASE
3. Acrosome Reaction / ZP penetration
- outer acrosomal membrane fuses w/ sperm membrane
- requires intracellular Ca rise, ZP3 binding, and Cδ4
- acrosin release not requried but helps disperse other proteases
- ZP2 binding keeps sperm in place
4. Membrane Fusion
- mediated by egg integrin +
sperm fertilin (ADAM-family fusion protein)
5. Cortical Reaction
- calcium waves release cortical granules triggered by PLCζ
(in sperm)
6. Block to Polyspermy
7. Egg Activated, Meisois 2 completes, Pronuclei (male and female)
form
- sex is now determined
- egg is metabolically active
- diploidy restored
Corona Radiata
Catsper
ZP3
Cδ4
acrosin
ZP2
integrin
fertilin
PLCζ
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