MCB 3020C Lecture Notes - Lecture 20: International Genetically Engineered Machine, Nostoc Punctiforme, Artemisia Annua
Document Summary
12. 11 from synthetic metabolic pathways to synthetic cells. Synthetic biology: using genetic engineering to create novel biological systems out of biobricks: example: international genetically engineered machine undergraduate competition. Engineering a major food product: vanillin is a secondary metabolite extracted from seedpods, engineered escherichia coli and yeast can synthesize vanillin from glucose. In e. coli five heterologously expressed enzymes are required to make. Synthetic cells: synthetic bacterium : artificial construction of a bacterial genome inserted into a different species to yield viable cells. Dna: also required protospacer adjacent motif, homologous recombination can be used to incorporate new dna, nonhomologous double-stranded dna break repair pathway can ligate after deletion. Crispr editing in practice: example: tomato mutation leads to needle-like or wiry leaves, example: targeting hiv or other viral rnas, example: removal of 62 copies of porcine endogenous retrovirus from swine cells to facilitate organ transplants.
