BIO E105 Lecture Notes - Lecture 13: Polymerase Chain Reaction, Dna Profiling, Complementary Dna
Document Summary
Research and discovery of gene function for use in genetic engineering: to alter gene expression in an organism of interest for medical, agricultural, scientific or industrial purposes. The steps of polymerase chain reaction: a reaction mix containing dntps, a dna template, copies of the two primers, and taq (dna polymerase), denaturation heating the mixture to 95 c separates the two strands of the. Dna profiling (also called dna testing, dna typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their unique dna profiles. One major method of dna profiling used today is based on pcr and uses short tandem repeats (str). In north america, a group of 13 sites for strs in human dna are used to generate a. Dna profile for a given individual: each individual will have a given allele for an str at a given site in their dna.
