CHE E122 Lecture Notes - Lecture 7: Epitestosterone, Dihydrotestosterone, Dehydroepiandrosterone
Document Summary
Functional groups are only hydroxyl and ketones. Trimethylsilyl iodide is used to catalyse the derivation: gives otms derivatives, gives single enoltms derivatives. Full scan mass spectrometry is another: scans a continuous set of masses determined by the operator, time of collection depends on the scan range, full spectral data so it"s good as a start. Steroid analysis uses sim ms for screening. Three ions are monitored for each substance. Maximum of 20 ions at a time to keep between scans short up to 10 such groups. It is important that the chromatography is done well but run time also needs to be short. Endogenous steroids occur in each individual and are excreted in the urine. Can be natural or synthetic - we need a way to distinguish between natural and synthetic in drug testing. It"s a lot more difficult to detect abuse of endogenous (natural) than exogenous (synthetic).
