BIOL 155 Lecture Notes - Lecture 7: Electrophoresis, Insulin, Plasmid
Document Summary
The evolution of gene manipulation: humans have been using selective breeding for centuries to manipulate genes. Dna technology: dna contains information, editing the information is dna technology, advances in dna technology allow, understanding of mechanisms of life, solving crimes, creating solutions in healthcare. Pcr has revolutionized many fields of biology: scientific research in medicine, diagnosis of genetic defects, detection of aids virus (hiv) in human cells, research in evolution, amplify trace amounts of dna from fossils. Forensic science: amplify trace amounts of dna from crime scenes. Electrophoresis: separates dna molecules based on size, dna has an overall negative charge, electrical current forces dna molecules to travel through the gel, small molecules move faster through the gel matrix than big molecules. Restriction enzymes: molecular scissors , proteins that recognize a specific dna sequence and cuts the dna helix, leaves sticky ends , can join sticky ends from difference pieces of.
