BIOSC 0100 Lecture Notes - Lecture 5: Maurice Wilkins, X-Ray Crystallography, Francis Crick

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Composed of a phosphate group, a five-carbon sugar, and a nitrogenous base. Sugar in rna is ribose (two oh) and in dna it is deoxyribose (one oh) Deo(cid:454)(cid:455)ri(cid:271)ose is (cid:373)issi(cid:374)g a h(cid:455)dro(cid:454)(cid:455)l group o(cid:374) the (cid:1006)". The phosphate group atta(cid:272)hes at the 5" a(cid:374)d the (cid:374)itroge(cid:374)ous (cid:271)ase atta(cid:272)hes at the (cid:1005)". Primary: sequence of nucleotides on the sugar-phosphate backbone. Pyrimidines 6 atom ring structure with c and n in ring. Purines 9 atom ring structure with c and n in the ring. Cytosine, urasil (rna), thymine (dna) [must know structure] cutie pye. Guanine, adenine [must know structure] pure as gold. Same as amino acids condensation reactions (monomer in, water out) Nucleotide pol(cid:455)(cid:373)erizatio(cid:374) for(cid:373)s phosphodiester (cid:271)o(cid:374)ds (cid:894)li(cid:374)kages(cid:895) (cid:271)et(cid:449)ee(cid:374) the (cid:1007)" c h(cid:455)dro(cid:454)(cid:455)l o(cid:374) o(cid:374)e (cid:374)u(cid:272)leotide a(cid:374)d the 5"c phosphate of the (cid:374)e(cid:454)t (cid:374)u(cid:272)leotide. The e(cid:374)erg(cid:455) for pol(cid:455)(cid:373)erizatio(cid:374) (cid:272)o(cid:373)es fro(cid:373) (cid:374)u(cid:272)leoside triphosphates (cid:894)(cid:862)a(cid:272)ti(cid:448)ated(cid:863) (cid:374)u(cid:272)leotides(cid:895) Used x-ray crystallography to determine that dna had a repeating, helical shape.

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