11:680:390 Lecture Notes - Lecture 6: Sanger Sequencing, Ion Semiconductor Sequencing, Horizontal Gene Transfer
13 Mar 2018
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Why do we study genomes, practical applications. 6: microbial systems biology: transcriptomics, proteomics, metabolomics: interactive mapping and predictive modeling; identifying potential drug targets, can be used for strain identification: monitoring disease outbreaks, diagnostics: detecting pathogens or determining types of microbes in an. Identifying microbial phyla: first genomes sequenced were small viruses. Microbial genomes: genome size comparison, extremely small genomes are endosymbionts, most small genomes involves parasites, 5 symbiont genomes are very small compared to mycoplasma (parasitic) genome, dna sequencing, first generation: sanger sequencing. Small amounts of ddntps used with dntps. Ddntps are chain terminators preventing further elongation of. Originally used radioactivity, but automated systems use fluorescent labels. ** when a new nucleotide is incorporated into the newly synthesized dna chain by dna polymerase, pyrophosphate (pp i ) and h 2 is released: second generation: pyrosequencing. Interrogates the template in a sequential manner in a known sequence. When bond is made, pyrophosphate is released (sulfurylase converts amp + ppi into atp)
