BIO 314 Lecture Notes - Lecture 2: S Phase, Insertional Mutagenesis, Myc
Document Summary
Chapter 3: tumor viruses: know what peyton rous did, and why it was significant. Took sarcoma from chicken and ground it up to put it into a healthy chicken, and discovered a chicken sarcoma virus in the tumors. Rous sarcoma virus infected a monolayer of chicken embryo fibroblasts in culture. Host cells didn"t die, but behaved like cultured cancer cells, called foci. Led to greater understanding of genes that could be altered to cause cancer in humans, known as oncogenes: know the properties of transformed cells (foci) Anchorage independent and grow on top of each other. High saturation density (accumulate large number of cells in dish). Inability to halt proliferation in response to growth factor deprivation. Normal cells are anchorage dependent and contact inhibited. They form a confluent monolayer: understand how retroviruses can transform cells. Retrovirus- virus integrates into the host cell dna. Forces over-expression of oncogenes that encode proteins with transforming properties.
