BSC 310 Lecture Notes - Lecture 12: Chemiosmosis, Cellular Respiration, Electrochemical Gradient

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24 Jun 2018
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Chemical Reactions and Energy
Microbial life can exist only where molecules and cells remain organized, and energy is
needed by all microorganisms to maintain organization.
Every activity taking place in microbial cells involves both a shift of energy and a
measurable loss of energy. Although the second law of thermodynamics says that energy
cannot be created or destroyed, but only transferred within a system, unfortunately, the
transfers of energy in living systems are never completely efficient. For this reason,
considerably more energy must be taken into the system than is necessary to simply carry
out the actions of microbial life.
In microorganisms, most chemical compounds neither combine with one another
automatically nor break apart automatically. A spark called the energy of activation is
needed. The activation energy needed to spark an exergonic (energy-yielding) reaction or
endergonic (energy-requiring) reaction can be heat energy or chemical energy. Reactions
that require activation energy can also proceed in the presence of biological
catalysts. Catalysts are substances that speed up chemical reactions but remain unchanged
during the reactions. Catalysts work by lowering the required amount of activation energy
for the chemical reaction. In microorganisms, the catalysts are enzymes.
Enzymes. Chemical reactions in microorganisms operate in the presence of enzymes.A
particular enzyme catalyzes only one reaction, and thousands of different enzymes exist in a
microbial cell to catalyze thousands of different chemical reactions. The substance acted on
by an enzyme is called its substrate. The products of an enzyme-catalyzed chemical reaction
are called end products.
All enzymes are composed of proteins. When an enzyme functions, a key portion of the
enzyme called the active site interacts with the substrate. The active site closely matches
the molecular configuration of the substrate, and after this interaction has taken place, a
shape change at the active site places a physical stress on the substrate. This physical
stress aids the alteration of the substrate and produces the end products. After the enzyme
has performed its work, the product or products drift away. The enzyme is then free to
function in the next chemical reaction. Enzyme-catalyzed reactions occur extremely fast.
With some exceptions, enzyme names end in “-ase.” For example, the microbial enzyme
that breaks down hydrogen peroxide to water and hydrogen is called catalase. Other well-
known enzymes are amylase, hydrolase, peptidase, and kinase.
The rate of an enzyme-catalyzed reaction depends on a number of factors, including the
concentration of the substrate, the acidity of the environment, the presence of other
chemicals, and the temperature of the environment. For example, at higher temperatures,
enzyme reactions occur more rapidly. Since enzymes are proteins, however, excessive
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Document Summary

Microbial life can exist only where molecules and cells remain organized, and energy is needed by all microorganisms to maintain organization. Every activity taking place in microbial cells involves both a shift of energy and a measurable loss of energy. Although the second law of thermodynamics says that energy cannot be created or destroyed, but only transferred within a system, unfortunately, the transfers of energy in living systems are never completely efficient. For this reason, considerably more energy must be taken into the system than is necessary to simply carry out the actions of microbial life. In microorganisms, most chemical compounds neither combine with one another automatically nor break apart automatically. A spark called the energy of activation is needed. The activation energy needed to spark an exergonic (energy-yielding) reaction or endergonic (energy-requiring) reaction can be heat energy or chemical energy. Reactions that require activation energy can also proceed in the presence of biological catalysts.

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