BIO 198 Lecture Notes - Lecture 20: Hfr Cell, Antimicrobial Resistance, Homologous Recombination
15 Oct 2016
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F plasmid: f factor small molecule that replicates independently of the chromosomal dna. Some genes are needed for the replication (proteins that ensure the distribution of the plasmids is distributed to the each daughter cells. Transfer origin where plasmid proteins build and then there is a break in the dna. Dna is sent into the other cell and copied into a double helix. (x conjugated: new cell recipient of the. It will transfer whatever dna is connected to it (anything that is attached to orit will get sent over). Homologous recombination, (between e coli and plasmid dna). There are pie(cid:272)es of dna that do(cid:374)"t perfor(cid:373) a(cid:374)y fu(cid:374)(cid:272)tio(cid:374) of the host dna (sometimes the insertion dna will jump into the plasmid dna). You"ll see more transfers of earlier markers than later markers. Almost never does a transfer of an integrated f become a donor cell that can donor the f (or become new donors)
