BIO SCI 97 Lecture Notes - Lecture 12: Xeroderma Pigmentosum, Intron, Epistasis
11 Sep 2017
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Attach to e. coli or yeast and then stay on them. Illegitimate recombination integrates introduced dna into random location of the genome. Homologous recombination requires homology between the dna and the site of insertion at nonrandom locations. Transgenic mice can be created through either illegitimate or homologous. Integration of transgene is used to knock out endogenous locus. Knock-out is used for reverse genetics (have sequence and unknown function) Knock out is replacing a gene (in mice) with the gene you have and finding out what the function of that new inserted gene is. Use of reverse genetics (known sequence, unknown phenotype) Reporter genes are used to find gene function. Take the enhancer/promoter and hook it up for gfp to investigate gene regulation. Fine-scale mutations = involving less than 1000 base pairs. Clicker question: b) sickle cell mutation is an example of missense mutation. Silent: changes a codon, but not the encoded protein.
