LIFESCI 3 Lecture Notes - Lecture 10: Dideoxynucleotide, Chain Termination, Str Analysis
Document Summary
Pcr: polymerase chain reaction, make copies of specific pieces of dna & amplifying them. Dna: pay attention to polarity (5-3, denature -> primer hybridization -> elongation, q: concentration of primers decreases/increases/stays the same with every cycle of pcr. Isolate dna samples: recover dna specimen from white blood cells (not red blood cells), semen, skin, lip prints, saliva, but that"s likely to (cid:271)e (cid:374)ot e(cid:374)ough. Make copies of specific portions of dna: to make more dna, use pcr, amplifies specific regions of dna for comparison between individuals. For dna profiling, short tandem repeats (strs) are used. Analyze & compare amplified dna: dna visualized using gel electrophoresis. Short tandem repeats (str: stretches of dna that are repetitive, strs are usually highly variable between different people (that are unrelated) in their lengths. A single str may differ between two unrelated people in only length. These repeats occur in the same place in the genome: str analysis.
