MCDB 20 Lecture Notes - Lecture 2: Rna Splicing, Tata Box, Transfer Rna

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olivecicada965

4 Dec 2017

School

UCSB

Department

Molecular, Cellular & Develop. Biology

Course

MCDB 20

Professor

Hui Low

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Document Summary

-aass rrnnaa ppoollyymmeerraassee mmoovveess aalloonngg tthhee ddnnaa,, iitt uunnttwwiissttss tthhee ddoouubbllee hheelliixx,, 1100 ttoo 2200 bbaasseess aatt aa ttiimmee. -iinn bbaacctteerriiaa,, tthhee ppoollyymmeerraassee ssttooppss ttrraannssccrriippttiioonn aatt tthhee eenndd ooff tthhee tteerrmmiinnaattoorr aanndd tthhee mmrrnnaa ccaann bbee ttrraannssllaatteedd wwiitthhoouutt ffuurrtthheerr mmooddiiffiiccaattiioonn. -iinn eeuukkaarryyootteess,, rrnnaa ppoollyymmeerraassee iiii ttrraannssccrriibbeess tthhee ppoollyyaaddeennyyllaattiioonn ssiiggnnaall sseeqquueennccee ((aaaauuaaaaaa));; tthhee rrnnaa ttrraannssccrriipptt iiss rreelleeaasseedd 1100--3355 nnuucclleeoottiiddeess ppaasstt tthhiiss ppoollyyaaddeennyyllaattiioonn sseeqquueennccee. -eennzzyymmeess iinn tthhee eeuukkaarryyoottiicc nnuucclleeuuss mmooddiiffyy pprree--mmrrnnaa ((rrnnaa pprroocceessssiinngg)) bbeeffoorree tthhee ggeenneettiicc mmeessssaaggeess aarree ddiissppaattcchheedd ttoo tthhee ccyyttooppllaassmm. -dduurriinngg rrnnaa pprroocceessssiinngg,, bbootthh eennddss ooff tthhee pprriimmaarryy ttrraannssccrriipptt aarree aalltteerreedd. -aallssoo,, uussuuaallllyy ssoommee iinntteerriioorr ppaarrttss ((iinnttrroonnss)) ooff tthhee mmoolleeccuullee aarree ccuutt oouutt aanndd tthhee ootthheerr ppaarrttss ((eexxoonnss)) sspplliicceedd ttooggeetthheerr. -tthhee nnoonnccooddiinngg rreeggiioonnss aarree ccaalllleedd iinntteerrvveenniinngg sseeqquueenncceess,, oorr iinnttrroonnss. -tthhee ootthheerr rreeggiioonnss aarree ccaalllleedd eexxoonnss aanndd aarree uussuuaallllyy ttrraannssllaatteedd iinnttoo aammiinnoo aacciidd sseeqquueenncceess. -rrnnaa sspplliicciinngg rreemmoovveess iinnttrroonnss aanndd jjooiinnss eexxoonnss,, ccrreeaattiinngg aann mmrrnnaa mmoolleeccuullee wwiitthh aa ccoonnttiinnuuoouuss ccooddiinngg sseeqquueennccee.

Related Questions

38. Which bacterial RNA polymerase synthesizes tRNA?

a. RNA polymerase I

b. RNA polymerase II

c. RNA polymerase III

d. Core enzyme

e. Promoter

39. In what part of the transcription process do transcription factors participate?

a. the binding of RNA polymerase to the DNA template

b. transcription initiation

c. elongation

d. termination

e. all of the above

periwinklehorse209

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

5. Which of the following statements regarding eukaryotic transcription is FALSE?

a. RNA polymerase II has a critical carboxy-terminal domain that is phosphorylated during

active transcription.

b. General transcription factors are designated TFII followed by a number, and they function in initiation of transcription across the eukaryotic genome.

c. The most highly transcribed eukaryotic genes contain CpG islands upstream of their transcription start site rather than TATA boxes.

d. Distal enhancers, like promoter-proximal elements, are composed of several small, 6â10 bp functional sequence elements.

MCDB 20 Lecture Notes - Lecture 2: Rna Splicing, Tata Box, Transfer Rna

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