ANFS332 Lecture Notes - Lecture 9: Polymerase Chain Reaction, Kary Mullis, Dna Profiling

• School: University of Delaware
• Department: Animal and Food Sciences
• Course: ANFS332
• Professor: Keeler Jr.Calvin

Document Summary

Polymerase chain reaction (pcr: a (cid:396)e(cid:448)olutio(cid:374)a(cid:396)(cid:455) (cid:373)ethod de(cid:448)eloped (cid:271)(cid:455) ka(cid:396)(cid:455) mullis i(cid:374) the (cid:1005)(cid:1013)(cid:1012)(cid:1004)"s. It"s a fast a(cid:374)d i(cid:374)e(cid:454)pe(cid:374)si(cid:448)e te(cid:272)h(cid:374)i(cid:395)ue used to a(cid:373)plif(cid:455) o(cid:396) (cid:272)op(cid:455) s(cid:373)all seg(cid:373)e(cid:374)ts of. Dna (thermocycler: this repetitive process can result in the creation of a billion copies of the original. Dna: o(cid:374)e (cid:272)op(cid:455) (cid:272)a(cid:374)"t (cid:271)e dete(cid:272)ted (cid:271)ut a (cid:271)illio(cid:374) (cid:272)a(cid:374, the technique is used for dna fingerprinting, detection of bacteria and viruses, and the diagnosis of genetic disorders. Sporulation: the (cid:449)o(cid:396)d (cid:862)spo(cid:396)e(cid:863) is de(cid:396)i(cid:448)ed f(cid:396)o(cid:373) the g(cid:396)eek (cid:449)o(cid:396)d fo(cid:396) seed, sporulation is initiated at the end of the growth period for some bacteria when nutrients become limited or other unfavorable conditions. The total time required for the completion of sporulation varies w/ the bacterial species and strain (7-10 hours) Spores: composed of a spore coat, a cortex, and a nuclear coat, they are produced intracellularly, they are refractile (they scatter light, spores are produced by gram + rods.