Chloe Evetts (8990-4674)
June 9, 2013
The purpose of this experiment is to learn how to examine a fecal sample for
eggs, cysts, ameba and other indicators of parasitic infections. This is a very
important procedure to ensuring the health of our animals. Parasites can be very
harmful to pets, especially if they are not identified early on. By learning this
procedure, we can ensure that our own pets are not hosts for a variety of
Parasites are extremely common, especially in Florida. There is such a variety of
different organisms that thrive in the hot and humid environment. Even with
routine worming, it is difficult to rid an animal of all of its parasites. I expect that
this experiment will show parasites present in the sample provided.
1. Take a small scoop with a wooden tongue depressor and mix with about 8 ml of
your flotation solution in a small bowl to make a homogeneous suspension.
2. Strain the dispersed fecal matter through a cheesecloth and funnel into a 10 ml
3. Fill the tube up to the top with the fecal solution using a transfer pipette. Make
sure the solution has a convex surface.
4. Put the cover slide on top of the tube/solution (it may spill a bit).
5. The cover slip has to contact the solution surface completely.
6. Let it sit for 1020 minutes.
7. The eggs will rise to the top and stick to the glass.
8. Lift up the cover slip vertically and put onto a glass slide.
9. Look for parasite eggs, cysts and other life forms with the 10x magnification.
No abnormalities were found in the sample taken from my personal dog, Molly. After
completing the procedure, there were no eggs, cysts or other life forms visible at the 10x
Molly is on routine parasite protection, and was recently given prevention within the past
week. Although it is possible that she could still be infected, it is unlikely. This
experiment showed that keeping pets up to date with parasite prevention is very helpful and important. Learning to perform this procedure is extremely advantageous, especially
because I am studying to be a veterinarian