MCB 150 Lecture Notes - Lecture 16: Enzyme, Central Dogma Of Molecular Biology, The Sequence
6 Mar 2017
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Amplification: this is done to create multiple copies of a gene that may only be present in 1 or 2 places in a cell. This explains why rna is made as an intermediate step in the central dogma of bio. For purposes of mcb, we will be working with transcription of e. coli. Has 6 subunits , together called a holoenzyme. Sigma can detach, when this happens the remaining 5 subunits are called a core enzyme. Only transcribes a specific gene (useful info) The start sequence of bps to start transcription. Look at slide 2 on lecture casts. The start point is given the name +1 base. This is the first base in transcription. Everything following the transcription direction (downstream) is +2,+3, ect. Vice versa for the direction going to the beginning of the promoter. 10 and -35 cite are conserved (hot spots) in e. coli. Conserved: a sequence is pretty much the same in all cells.
