DNA Sequencing (Sanger method):
https://www.dnalc.org/resources/animations/sangerseq.html
Questions:
5. In what ways are PCR and Sanger sequencing similar?
6. What is the important difference in Sanger sequencing?
7. Why is size separation of DNA fragments so important in Sanger sequencing? How are the DNA fragments separated in the animation?
Additional videos on Sanger DNA sequencing:
Sanger Sequencing
https://www.youtube.com/watch?v=6ldtdWjDwes
The Sanger Method of DNA Sequencing
https://www.youtube.com/watch?v=FvHRio1yyhQ
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Bacterial Transformation:
https://www.dnalc.org/resources/animations/transformation1.html
Questions:
8. What are plasmids?
9. What was used to cut the plasmids?
10. What was used to seal the DNA fragments together?
11. How did the researchers select for bacteria that contain both antibiotic resistance genes?
12. What is a recombinant plasmid?