MCB 250 Lecture Notes - Lecture 4: Restriction Enzyme, Recognition Sequence, Ecori
9 Jan 2020
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Why restriction endonuclease: bacteria possess enzymes that restrict incoming foreign dna (like bacteriophage dna) by cleaving it. Dna separation by gel electrophoresis: purposes, analytical tool. To identify or learn something about a particular piece of dna: size, topology, restriction pattern, purity, concentration: preparative tool. Southern blots: ethidium bromide stains all dna fragments, southern blotting allows us to identify a fragment that carries one particular gene. Northern blots: similar procedure to southern blots, but can be used to identify a particular mrna from a population of. Coomassie-stained sds-page gel: sds-page can be used to separate proteins by size. Coomassie-blue dye binds to proteins for visualization of bands: as with dna, the mobility of any given protein in an. Sds-page gel depends on: charge density, shape, size (mw, migration is inversely proportional to the log10(mw), with larger proteins on top, smaller proteins on bottom.
