BIOS 111 Lecture Notes - Lecture 22: Fluorescence Microscope, Merck Millipore, Bactericide
Document Summary
Millipore filter: fibers spun in a sheet so we can get a variety of sizes. Nuclepore: filter is solid but we shoot radiation through to make pores. The type of radiation determines the pore size. Hospitals, burn wards, and operating rooms have filtered air. Military ships and tanks have filtered air. Semiconductor and pharmaceutical industries have filtered air. To determine amount, we collect the filter and count the microbes on them. We place the filter on a certain type of plate and then count the colonies. This normally takes 2 days to get the information. We will take the filter and stain it with a fluorescent dye then look at it under fluorescent microscopy so we can look at the bacteria within 10-15 minutes. Commercial sterilization: what the canning industry does. Antisepsis: we do the disinfection on living human tissue. Degerming: removes microbes from a limited area such as an injection site. Freezing can kill in a freeze-thaw cycle.
