CHEM 451 Lecture Notes - Lecture 3: Thioester, Ubiquitin, Transesterification

8 views3 pages
School
Department
Course
Professor

Document Summary

Hect example on e3 ligase in alanine scanning. We mentioned in class that there are 3 enzymes that are important for ubiquitination of a substrate - e1, e2, e3. Steps that occur is that each enzyme (e1 and e2) have a free cysteine that is the reactive group. Thioester bond = high energy bond, this is how the energy of the atp gets transferred into the. Transesterification = transferring from one thioester to a second thioester. E2 comes in and it"s able to transfer the ubiquitin from the first thioester on e1 to the thioester on e2 -> this is where you get differences in what happens next. Hect domain e3 = transfer of the ubiquitin first to e3 and then to the substrate. Second path is illustrated by the ring domain e3 -> direct transfer of the ubiquitin onto the substrate (within the context of the e3 ligase)

Get access

Grade+
$40 USD/m
Billed monthly
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers

Related textbook solutions

Related Documents

Related Questions