CHEM 451 Lecture Notes - Lecture 13: Signal Recognition Particle, Translocon, Cell Membrane
Document Summary
Tm7 and tm2 are the ones that form initial interaction with signal sequence. Cross linking mass spec where they stopped proteins being translocated in protein and cross linked to see what connections were being made. Critical for directing protein to membrane and orietning it correctly. Even when things get cleaved off ,everything ends up where it should. Little pink rod represents signal sequence of protein that has been translocated into translocon. In transmembrane 2 and 7 have been determined to be important when interacting with helix. Transmembrane helix forms a short helix and link with helix depends upon actual sequence. Leaves the pore open to continue to translate. Back on the right there"s a hinge motion, at some point, the signal is to stop translating and allow for insertion, the whole things opens up and partition into membrane. Blue or red represent hydrophobic regions of translocon. Net positive charge / net negative charge along.
