CHEM 451 Lecture Notes - Lecture 14: Titin, Ribbon Diagram, Morphism Of Algebraic Varieties
Document Summary
On the right = sequential unfolding of proteins, comparing extensions measured and force needed to unfold it. Look at multidomain protein and looking at folding and unfolding of it. Protein they chose to study was titin, related with muscle elasticity (expands and contracts regularly. Titin = protein of muscle cell, links __ to z disc. Choosing it because it"s a protein that unfolds as part of its regular function, so it"s important that it refolds correctly. They contrast this to proteins that fold cotranslationally. Proteins that may live for a very long time (avg lifetime of protein given some sort of stability of protein when would we expect it to unfold) ____ Premise that there are unfolded states and they want to understand it. Sequence of adjacent titin domains = clear hypothesis, look at them adjacent domains that have less sequence similarity -> important for preventing misfolding.
