CHEM 451 Lecture Notes - Lecture 20: Threonine, Eif4Ebp1, Beta Sheet
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Highlights that there are 2 sites (thr 37 and thr 46) were critical. Phosphothreonine 46 below, phosphate makes h bonds with residues near it. Phosphomimics didn"t work becausei t oculdn"t make the ___ Couldn"t make h bonds that stabilised turn regions. Other important part is the tyr 54, this gets phosphorylated. Green strand that forms helix/ beta strand also contains site gets ubiquitinated when protein gets degraded. Ubiquitination site is sequestered from ____ in the folded beta sheet structure. This is why protein is much more stable. Space filling model that phosphothreonine is more ____ Slide 4 phospho regulation of the eif4e:4e-bp interaction. Summary of other phosphorylation sites outside of the ones showed in the previous slide. 37 and 46 = two magnitude loss in ___ In the pwt one shows ____ factor of 10 or 20 from the additional phosphorylation. In direct parallel to kinase, it"s a dynamic loop so ____ and exposed regioni, so residues may be phosphorylated.
