L07 Chem 481 Lecture 18: Lecture 18
5 Mar 2017
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Document Summary
The enzyme is called a mutase when a phosphate is moved from 1 carbon to another as occurs with phosphoglycerate mutase that converts 2-phosphoglycerate to 3-phosphoglycerate. A mutase is an enzyme of the isomerase class that catalyzes the shifting of a functional group from one position to another. Metabolic flux through glycolysis increases by ~100 fold between rest and rigorous exercise in skeletal muscles. Metabolite effectors (ms) < 2nd messengers (hormones) (seconds) < genes (hours) Atp negatively regulates pkf1 (reduces f6p -> f-1,6p) [atp] varies by <10% insufficient to get 100-fold change. Because of the large proportional difference, small changes in amp greatly increases the [amp] making it a huge signal. 10% change in [atp] 500-600% change in [amp] Only gives up to about 10x change in flux, but doesn"t account for the 100x change in flux. F6p + atp -(pfk-1)> f1,6-bp + adp -(f1,6-bpase)> f6p + pi. Low flux = v_f (min) - v_r (max)
