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) The following data were obtained from a thermal resistance experiment conducted on a spore suspension at 113oC. Determine the DT value of the microorganism (4 min).
Time (min) | Number of spore survivors |
0 | 106 |
4 | 1.05 x 105 |
8 | 1.08 x 104 |
12 | 1.03 x 103 |
) For the same spore suspension, a set of DT was measured at different temperatures, as shown below. Determine the thermal resistance of the spore, z value in oC (11oC).
Temperature (°C) | D (minutes) |
104 | 27.5 |
107 | 14.5 |
110 | 7.5 |
113 | 4.0 |
116 | 2.2 |
3-c) A sterilizer is used to obtain a 12-log reduction of the same spore in a juice product with an initial load of 5.7 x 108 spores per mL of product. How long would it take to achieve the 12 log reduction at the following temperature: a) 116 °C and b) 121oC; c) what is the final concentration of spores per liter? d) what is F0? (26.4 min; 9.3 min; 0.57/L; 9.3 min)