CMB Lecture Notes - Uncompetitive Inhibitor, Cuvette, Enzyme Kinetics

In biology studies, if we have any colored solutions, that means we can measure the INTENSITY of the COLOR by spectrophotometry and relate it to the concentration of that component (solute) at any given time. TRUE OR FALSE? Explain.

What is the range of numbers for absorbance in spectrophotometry? __ to ____ and What number is beyond limit?

Explain the relationship between optical density and absorbance value.

How could you use the spectrophotometer to determine the starch concentration in the two test tubes in terms of g/L?

Assume that you measure the OD of two E.coli cultures: culture A and culture B. The spectrophotometer gives you a reading of 3.1 for culture A and 0.03 for culture B. What would you do to determine the concentration of culture A and B and why? Give a detailed explanation!