BIOL 1107 Study Guide - Semiconservative Replication, Dna Replication, Primase
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Three proposed models for replication: semiconservative replication, conservative replication, dispersive replication. Important enzymes to remember: dna polymerase iii and dna polymerase i, dna helicase, dna ligase, single-stranded dna-binding proteins (ssbps, topoisomerase, primase. Key locations/terms: origin of replication, replication fork, dntps, leading strand (continuous) v. lagging strand (discontinuous) Steps: dna is opened, unwound and primed, key players: dna helicase, ssbps, topoisomerase, primase, synthesis of the leading strand begins, key players: dna polymerase, sliding clamp. Dna replication - synthesis of the leading strand. Steps: primer is added, key players: primase, first fragment is synthesized, key players: dna polymerase iii, sliding clamp i. Forms okazaki fragments: second fragment is synthesized, key players: primase, dna polymerase iii, sliding clamp, primer is replaced, key players: dna polymerase i, gap is closed, key players: dna ligase. When replication form reaches the end of a linear chromosome at the lagging strand, primase adds and rna primer close to the end of the chromosome.
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