A local pesticide-producing plant has been leaking high concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) into a cellulose-rich soil for years. In an attempt to reduce the 2,4-D concentrations in the soil, scientists found that 2,4-D degrading microorganisms were already present in the soil.
(a) Explain how the presence of 2,4-D degrading microorganisms was determined. Individual steps do not need to be provided but explain the method/approach and why this would provide the needed information.
(b) Taking into consideration the nutrients required for microbial growth in general, provide two reasons why the cell yield on 2,4-D might be low?
(c) Of the metabolisms we discussed (aerobic/anaerobic respiration, fermentation, aerobic/anaerobic chemolithotrophy, oxygenic/anoxygenic photosynthesis), predict the dominant metabolism and explain why you expect this metabolism to be most abundant.
(d) Could other metabolisms be present? If yes, explain what other metabolisms are present and why they are present. If no, explain why other metabolisms are not present.