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Midterm

BIOL 459 Midterm: Classification of Infectious Agents- Viruses

4 Pages
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Department
Biology
Course Code
BIOL 459
Professor
Roy Real

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[Classification of Infectious Agents: Viruses
Intro:
What are bacteriophages?
Viruses that infect bacteria
What are the structures of a virion
(completely developed viral particle)?
● Nucleic acid
Capsid
Envelope
Spikes
Morphology of Viruses (4).
● Helical hollow, cylindrical
● Polyhedral many-sided
Enveloped
● Complex  combine helical and
polyhedral
Taxonomy of viruses.
● Genus -virus
● Family - viridae
● Order - ales
Growing Bacteriophages in Laboratory
● Viruses  be grown in living cells!
● Bacteriophages grown in bacteria
○ Form plaques on the surface
of agar plate
○ Measured in plaque-forming
units (PFU)
Growing Animal Viruses in the Laboratory
● In living animals
● In embryonated eggs
○ Viruses injected into egg
● In cell cultures
○ Tissues treated w/ enzymes to
separate cells
○ Cytophatic effect→
deterioration of cell with virus
○ Continuous cell lines used
HeLa
Viral Identification
● Cytopathic effects
● Serological tests
● Nucleic acids
Name the two bacteriophage cycles.
● Lytic cycle
○ Phage DNA incorporated into
host

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Description
[Classification of Infectious Agents: Viruses Intro: What are bacteriophages? Viruses that infect bacteria What are the structures of a virion Nucleic acid (completely developed viral particle)? Capsid Envelope Spikes Morphology of Viruses (4). Helical hollow, cylindrical Polyhedral many-sided Enveloped Complex combine helical and polyhedral Taxonomy of viruses. Genus -virus Family - viridae Order - ales Growing Bacteriophages in Laboratory Viruses be grown in living cells! Bacteriophages grown in bacteria Form plaques on the surface of agar plate Measured in plaque-forming units (PFU) Growing Animal Viruses in the Laboratory In living animals In embryonated eggs Viruses injected into egg In cell cultures Tissues treated w/ enzymes to separate cells Cytophatic effect deterioration of cell with virus Continuous cell lines used HeLa Viral Identification Cytopathic effects Serological tests Nucleic acids Name the two bacteriophage cycles. Lytic cycle Phage DNA incorporated into host
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