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Midterm

BIOL 459 Study Guide - Midterm Guide: Alphavirus, Lentivirus, Provirus

4 pages65 viewsFall 2017

Department
Biology
Course Code
BIOL 459
Professor
Roy Real
Study Guide
Midterm

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[Classification of Infectious Agents: Viruses
Intro:
What are bacteriophages?
Viruses that infect bacteria
What are the structures of a virion
(completely developed viral particle)?
● Nucleic acid
Capsid
Envelope
Spikes
Morphology of Viruses (4).
● Helical hollow, cylindrical
● Polyhedral many-sided
Enveloped
● Complex  combine helical and
polyhedral
Taxonomy of viruses.
● Genus -virus
● Family - viridae
● Order - ales
Growing Bacteriophages in Laboratory
● Viruses  be grown in living cells!
● Bacteriophages grown in bacteria
○ Form plaques on the surface
of agar plate
○ Measured in plaque-forming
units (PFU)
Growing Animal Viruses in the Laboratory
● In living animals
● In embryonated eggs
○ Viruses injected into egg
● In cell cultures
○ Tissues treated w/ enzymes to
separate cells
○ Cytophatic effect→
deterioration of cell with virus
○ Continuous cell lines used
HeLa
Viral Identification
● Cytopathic effects
● Serological tests
● Nucleic acids
Name the two bacteriophage cycles.
● Lytic cycle
○ Phage DNA incorporated into
host
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