BIOL 2153 Study Guide - Final Guide: Repressor, Nucleic Acid Sequence, Lac Operon
Document Summary
Vibrio cholera: infectious gastroenteritis, ingesting contaminated food or water, infected patients can die within three hours, enter stomach(cid:224) v. cholera cells limit protein synthesis, move to small intestine (cid:224) bacteria secrete proteases to break down the mucosal lining, reach the intestinal epithelial cells (cid:224) stop making flagellin; make and secrete toxin, toxin depletes cells of chloride ions; secrete water; severe dehydration, all possible because of gene regulation. No rna polymerase activity (cannot get past repressor: describe what happens in each experiment in terms of lac operon, experiment 1 (glucose only) = grows much more efficiently (faster) in presence of glucose, experiment 2 (lactose only) = utilizing slower; having to break down lactose genetics and the lactose (lac) operon, experiment 3 (glucose and lactose) = stops when glucose is used then it initiates lac operon structural genes and begins breaking down lactose.
