MMG 301 Study Guide - Final Guide: Facultative Anaerobic Organism, Serial Dilution, Alkaliphile
Document Summary
The process of diluting a sample several times. A method of isolating a culture by spreading microorganisms over the surface of a solid culture medium. Stock cultures cultures of microorganisms maintained solely for the purpose of keeping microorganisms in a viable condition by subculture, as necessary, into fresh culture. Aseptic techniques refers to a procedure that is performed under sterile condition. Neutrophile a highly phagocytic granulocyte; also called polymorphoculear leukocyte (pmn). Not stained strongly or definitely by either acid or basic dyes but stained readily by neutral dyes. Alkaliphile microbe classified as extremophile that thrive in alkaline environments at ph 9-11. Obligate means "necessary" so obligate aerobes and anaerobes will need oxygen and lack of oxygen respectively. Facultative anaerobes do best without oxygen but can still get by in its presence. Microaerophiles is a microorganism that requires oxygen to survive, but requires environments containing lower levels of oxygen than are present in the atmosphere.